Journal: The Journal of allergy and clinical immunology

Article Title: Claudin-18 deficiency is associated with airway epithelial barrier dysfunction and asthma

doi: 10.1016/j.jaci.2016.02.035

Figure Lengend Snippet: IL-13 decreases claudin-18 expression in primary human airway epithelial cells. A) IL-13 decreased claudin-18 protein levels (representative blot shown, brackets indicate cells from the same donor)(n=8, *P<0.05 by paired analysis). B) IL-13 decreased claudin-18.1 mRNA levels, but did not significantly change claudin-1, -4 or -7 mRNA abundance (n=8, *P<0.05).

Article Snippet: Recombinant human IL-13 (R&D Systems) was reconstituted in sterile PBS containing 0.1% bovine serum albumin.

Techniques: Expressing

Journal: The Journal of allergy and clinical immunology

Article Title: Claudin-18 deficiency is associated with airway epithelial barrier dysfunction and asthma

doi: 10.1016/j.jaci.2016.02.035

Figure Lengend Snippet: IL-13 decreases claudin-18 expression in mice. A) Mice hemizygous for a transgene that overexpresses IL-13 (IL-13 TG) have lower claudin-18 protein (*P<0.05) and B) mRNA levels than wild type littermates.

Article Snippet: Recombinant human IL-13 (R&D Systems) was reconstituted in sterile PBS containing 0.1% bovine serum albumin.

Techniques: Expressing

Journal: iScience

Article Title: OBP2A regulates epidermal barrier function and protects against cytotoxic small hydrophobic molecules

doi: 10.1016/j.isci.2024.111093

Figure Lengend Snippet:

Article Snippet: Recombinant Human IL-13 Protein , R&D Systems , 213-ILB/CF.

Techniques: Imaging, Recombinant, Transfection, Saline, Sterility, Cell Culture, Protease Inhibitor, DC Protein Assay, Binding Assay, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Quantitation Assay, Fluorescence, Sequencing, Software